A bacterial sensor system based on Methylomonas flagellata AJ 3670 is
described for methane determinations. The system consists of a bacterial,
reactor, a reference reactor and two oxygen sensors.
The current decreases with time until a steady state is
reached within 30 s at 30°C; the maximum current difference is obtained at 30°C
and pH 7.2. The response time for the determination of methane is less than 1
min.
A linear relationship is obtained between the current
difference and the methane concentration below 6.6 mM; the lower limit of
determination is 5 μM, and the current decrease is reproducible within 5%. The
current output of the methane gas sensor is almost stable for more than 10 days and 250
assays.
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