The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system.
In control experiments, the degree of non-specific binding was
negligible. The method of detection was used to monitor the adsorption
of two other proteins, cytochrome c and glucose oxidase, on to the IDE
microwave sensor surface.
The response of the sensor was also examined on different substrate
materials, with detection of protein binding observed obtained on both
smooth, conductive (gold) and on rough, insulating (hydroxyapatite)
surfaces.
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